Objective To translate the Fear Towards Artificial Intelligence Scale (FtAIS) into Chinese and test its reliability and validity in undergraduate nursing students, and to provide a reliable tool for accurately assessing nursing students′ attitudes towards artificial intelligence. Methods Following the Brislin translation model, the scale underwent forward translation, back-translation, and cross-cultural adaptation, and a pre-survey was conducted to form the Chinese version of FtAIS. A total of 375 undergraduate nursing students were investigated by convenience sampling to test the reliability and validity of the scale. Among them, 175 samples were used for exploratory factor analysis (EFA), and 200 samples were used for confirmatory factor analysis (CFA). Results The Chinese version of FtAIS consisted of 10 items. EFA extracted two common factors, namely work-related concerns and humanity and ethics-related concerns, with a cumulative variance contribution rate of 78.958%. CFA showed that the model fitted well (χ2/df=1.482, RMSEA=0.049, IFI=0.986, TLI=0.981, CFI=0.985). The overall Cronbach′s α coefficient of the Chinese version of FtAIS was 0.918, and the Cronbach′s α coefficients of the two dimensions were 0.905 and 0.919, respectively; the test-retest reliability was 0.836. The scale-level content validity index (S-CVI) was 0.975, and the item-level content validity index (I-CVI) ranged from 0.875 to 1.000. Conclusion The Chinese version of FtAIS demonstrates good reliability and validity and can serve as an effective tool for assessing the level of fear towards artificial intelligence among undergraduate nursing students.