Objective To explore the effectiveness of end-of-life care scenario-based virtual simulation teaching in a death education course. Methods Based on real cases of disease progression and outcomes in terminally ill cancer patients from an oncology hospice ward, a virtual simulation teaching was designed, comprising six teaching scenarios:teaching introduction, admission care, specialty care, spiritual care, grief counseling, and postmortem care.Then virtual simulation-based experimental teaching practices in end-of-life care were conducted for 82 undergraduate students enrolled in the elective course "Education on Life and Death".Students′ attitudes toward end-of-life care were compared before and after the teaching, the experimental teaching effect and students′ acceptance of the virtual simulation teaching were also assessed. Results The students′ overall score for the experimental teaching was (87.24±6.58).After the experimental teaching, students′ attitudes toward end-of-life care were significantly improved (P＜0.05), and their acceptance rating of the experimental teaching was (5.32±0.55) points. Conclusion End-of-life care scenario-based virtual simulation for experimental teaching can positively influence students′ attitudes toward end-of-life care, and demonstrates high level of student acceptance.