Objective To sinicize the Nurse-Patient Therapeutic Interaction Scale (NuPTIS) and test its reliability and validity, so as to provide a standardized tool for evaluating the therapeutic interaction ability of clinical nurses in China. Methods With the authorization of the original scale author, forward translation, back-translation, and cross-cultural adaptation were conducted based on the Brislin′s translation model, and the Chinese version of the NuPTIS was formed through a pre-survey. In August 2025, a questionnaire survey was carried out among 459 clinical nurses from 4 Grade A tertiary hospitals in Zhenjiang, Changzhou, and Nanjing using a convenience sampling method to verify the reliability and validity of the scale. Results The Chinese version of the NuPTIS included 20 items, divided into 4 dimensions:emotional support and motivation (6 items), empathy and clear communication (5 items), patient-centered care (6 items), and supportive communication process (3 items). Exploratory factor analysis showed that the cumulative variance contribution rate of the 4 common factors was 75.599%. Confirmatory factor analysis indicated that, the model had a good fit (χ2/df=1.242, RMSEA=0.033, GFI=0.920, CFI=0.988, NFI=0.988, TLI=0.986). The overall Cronbach′s α coefficient of the scale was 0.929, and the Cronbach′s α coefficients of each dimension were 0.872-0.936; the test-retest reliability was 0.956; the scale-level universal agreement content validity index was 0.950, and the item- level content validity index of each item ranged from 0.875 to 1.000; the average variance extracted (AVE) of the 4 dimensions was 0.667- 0.708, and the composite reliability (CR) was 0.874-0.935, showing ideal convergent validity. Conclusion The Chinese version of the NuPTIS has good reliability and validity, which is suitable for evaluating the therapeutic interaction ability of clinical nurses in China, and can provide a reliable measurement tool for nursing quality improvement and relevant scientific research.