Objective To explore the effect of parent-participated animation intervention on preschool children undergoing intravenous placement in the emergency department, and to provide a reference for optimizing procedural pain management. Methods A total of 80 preschool children requiring venipuncture in the emergency department were randomly divided into a control group and an intervention group, with 40 in each group. The control group received routine care and parental companionship; the intervention group received a structured parent-participated animation intervention in addition to the routine care, specifically, parents guided their children to watch self-selected favorite cartoons from 5 minutes before the procedure until 5 minutes after its completion.Venipuncture pain scores, fear scores, procedural compliance, first-attempt success cases, procedure duration, parental concern level, and parental satisfaction with the procedure were compared between the two groups. Results The venipuncture pain score in the intervention group was significantly lower than that in the control group, and parental satisfaction was significantly higher (both P＜0.05).There were no statistically significant differences between the two groups in venipuncture fear score, procedural compliance, first-attempt success cases procedure duration, or parental concern level (all P＞0.05). Conclusion Parent-participated animation intervention can effectively reduce venipuncture pain in preschool children in the emergency department and improve parental satisfaction with the venipuncture procedure.