Objective To synthesize the best evidence on the application of less-invasive surfactant administration technique in preterm infants to guide clinical. Methods We searched guideline repositories, professional association websites, UpToDate, BMJ Best Practice, Joanna Briggs Institute (JBI), Cochrane Library, PubMed, Embase, CINAHL, Science Direct, SinoMed, CNKI，Wanfang Data, VIP，etc., to collect relevant articles form January 1, 2015 to April 5, 2025. Two researchers who had recieved evidence-based nursing training used validated tools to evaluate the quality of the literature and extracted evidence. Results A total of 12 articles were included, encompassing 1 clinical decisions,4 guidelines, 1 evidence summaries, 4 systematic reviews, 2 expert consensus reports. A total of 21 pieces of best evidence were collected, mainly involving 5 aspects of indicated population and contraindications, the timing of drug administration, material preparation, operation implementation, personnel management and training. Conclusion The best evidence summarized in this study on less-invasive surfactant administration for preterm infants can inform the development of clinical procedures and serve as a reference for standardizing clinical practice.