Objective To explore the effect of Artificial Intelligence (AI) combined with the DISC model in the training of nursing students′ humanistic cultivation and communication skills. Methods A total of 170 nursing students from 4 undergraduate classes enrolled in 2024 were selected and divided into a control group of 84 students from 2 classes and an intervention group of 86 students from the other 2 classes by using the lottery method.In the teaching of nursing humanistic cultivation and communication skills, the control group adopted an integrated teaching method combining group learning, problem-based learning, and scenario simulation teaching, while the intervention group additionally adopted AI combined with DISC （dominance, influence, steady, compliance） personalized teaching method. Results After the teaching, the scores of clinical communication ability, humanistic care ability and professional identity in the intervention group were significantly higher than those in the control group （all P＜0.05）. Conclusion In the teaching of nursing huma- nistic cultivation and communication skills training, implementing AI combined with DISC personalized teaching is beneficial for enhancing nursing students′ clinical communication skills, humanistic care abilities, and professional identity.