Objective To explore a medical-education integrated dual-subject cultivation model for pediatric nursing talent, and to evaluate its practical effectiveness, so as to provide a reference for cultivating pediatric nursing talent. Methods Based on a comprehensive review of relevant national policies and literature, and aligned with pediatric nursing workforce needs in Shenzhen, a medical-education integrated dual-subject cultivation model was developed over seven years through multiple rounds of nursing expert consultation.The model consisted of university-hospital collaborative cultivation, establishment of a blended full-time and adjunct faculty team, an integrated general-specialty curriculum, formation of pediatric nursing class, synchronized theoretical-practical instruction, and a diversified evaluation system.A comprehensive evaluation of cultivation outcomes was conducted using quantitative data from five cohorts of graduates, a student learning satisfaction survey, qualitative interviews with core faculty members, academic mentors, and nursing administrators, supplemented by relevant educational achievement materials. Results Graduates de-monstrated strong performance in theoretical and skills assessments, the average pass rate for the National Nurse Licensing Examination was 98.00%, and the pass rate for the 1+X skill certification was 100.00%.The employment rate reached 100.00%.Overall student learning satisfaction scored 4.34±0.89.In addition, the model produced multiple achievements in teaching reform, research innovation, educational resource development, and the establishment of practice-based training platforms. Conclusion The medical-education integrated dual-subject cultivation model is conducive to improving the cultivation effect of pediatric nursing ta-lent, and it is recognized by both graduates and employers.