Objective To explore the effect of implementing Standard Operating Procedures (SOP) for isolation technique in intrao-perative radiotherapy for breast-conserving surgery, and to provide a reference for improving the quality of isolation technique ope-rations in this context. Methods A total of 240 breast cancer patients undergoing intraoperative radiotherapy during breast-conserving surgery were selected and divided into an experimental group and a control group chronologically, with 120 cases in each group.The control group received routine isolation technique operations, while the experimental group additionally implemented SOP management for isolation technique in intraoperative radiotherapy. Results The total score for isolation technique operations among operating room nurses in the experimental group was (91.08±2.89), which was significantly higher than that in the control group (89.14±2.90).The incidence of radiation dermatitis at 1 month and 3 months after surgery in the experimental group were significantly lower than those in the control group (both P＜0.05).Additionally, the average radiation dose in the operating room and the average cumulative radiation dose for medical staff in the experimental group were significantly lower than those in the control group (all P＜0.05). Conclusion Implementation of SOP in isolation technique operations during intraoperative radiotherapy for breast-conserving surgery can significantly improve the standardization of operations by medical staff, reduce the incidence of postoperative radiation dermatitis in patients, and lower the risk of radiation exposure for medical staff.