Objective To investigate the current status and its influencing factors of artificial intelligence (AI) literacy among clinical nurses, and to provide a reference for developing AI training programs. Methods A total of 574 nurses from six grade A tertiary hospitals were selected using a convenience sample, then they were investigated by utilizing the Artificial Intelligence Literacy Scale, the Attitudes towards Artificial Intelligence Application at Work Scale, and the Nurse Job Crafting Scale.Pearson correlation analysis and multiple linear regression analysis were conducted to identify factors influencing nurses′ AI literacy. Results The participants′ total scores of AI literacy, attitudes towards AI, and job crafting were (59.14±9.48) points, (82.56±13.26) points, and (76.91±12.70) points, respectively.Pearson correlation analysis indicated that, AI literacy score had significantly positive correlations with the scores of attitudes towards AI and job crafting (both P＜0.05).Multiple linear regression analysis revealed that, the use of AI in clinical and research settings, educational background, understanding of algorithms, training expe-rience, availability of AI equipment and usage guidelines in the hospital, job position, job crafting, and attitude towards AI were the main influencing factors of nurses′ AI literacy (all P＜0.05), which collectively explained 56.3% of the total variance. Conclusion Nurses′ AI literacy is at a medium and above level, while their competencies in ethics and practical application are still weak.It is suggested nursing administrators to implement targeted interventions to enhance clinical nurses′ AI literacy according to the influencing factors.