Objective To explore the current status and its influencing factors of work withdrawal behavior among clinical nurses in grade A tertiary hospitals, so as to provide a reference for developing targeted interventions. Methods A total of 3,802 clinical nurses from 13 grade A tertiary hospitals in Anhui Province were selected using a convenience sampling method, then they were investigated by utilizing the Work Withdrawal Behavior Scale, the Challenge-Hindrance Stressor Scale, the Perceived Organizational Support Scale, and the Abusive Supervision Scale. Results The participants′ score of work withdrawal behavior was 25.00(21.00,30.00).Multivariate linear regression analysis revealed that, gender, working years, professional title, monthly performance pay, employment status, working department, current position, challenge-hindrance stressors, organizational support, and abusive supervision were main influencing predictors of nurses′ work withdrawal behavior (all P＜0.05), which accounted for 34.6% of the total variance. Conclusion Clinical nurses in grade A tertiary hospitals exhibit moderate and low levels of work withdrawal behavior, and it is influenced by demographic characteristics and work environment factors.It is recommended that nursing administrators optimize organizational support systems and improve stress management mechanisms according to the influencing factors, so as to reduce nurses′ work withdrawal behavior.