Objective To develop a questionnaire to assess inpatients′ satisfaction with the humanistic care of head nurses and test its reliability and validity. Methods The initial version of the questionnaire was developed based on Managerial Role Theory and the Successful Manager′s Role Model, using literature review, semi-structured interviews, and Delphi expert consultation. Then 520 inpatients were surveyed to test the reliability and validity of the questionnaire. Results The questionnaire consists of 3 dimensions and 27 items. Exploratory factor analysis extracted 3 common factors with eigenvalues greater than 1, accounting for 66.917% of the total variance. Confirmatory factor analysis indicated a good model fit(χ2/df=1.330, RMSEA=0.034, GFI=0.902，NFI=0.910，IFI=0.976，CFI=0.976). The scale-level content validity index (S-CVI) was 0.965, and the item-level content validity index (I-CVI) ranged from 0.813 to 1.000.The overall Cronbach′ s α coefficient was 0.950，and the split-half reliability was 0.791. Conclusion The Inpatients′ Satisfaction with Humanistic Care of Head Nurses Questionnaire is reliable and valid to assess patients′ satisfaction with humanistic care provided by head nurse in the ward.