Objective To evaluate the effects of homogenized nursing training in output hospitals of national regional medical centers based on the Kirkpatrick model.Methods A total of 90 training nurses from the national regional medical center were selected as research subjects, and 12 months of homogeneous nursing training were implemented in the output hospital.The training effects were evaluated based on the Kirkpatrick model from the reaction level, learning level, behavior level, and result level.Results Reaction level:the satisfaction scores of nurses for various aspects of the training were 4.74 to 4.90 (out of 5).Learning level:after the training, the theoretical examination and clinical practice operation scores of nurses were significantly higher than those before the training (all P＜0.05).Behavior level: after the training, the total score and scores of each dimension of the core ability of nurses were significantly higher than those before the training (all P＜0.05). Result level:the interview results of the head nurses and teaching teachers of the training department showed that the comprehensive ability of the trained nurses was improved, and the overall efficiency of the department was enhanced; the interview results of the trained nurses showed that the training was important, and their sense of identity and self-confidence in nursing work was enhanced. Performance data: during the training period, there were no nursing adverse events or patient complaints, and many nurses received various levels of honors and rewards.Conclusion The homogeneous nursing training carried out by the output hospitals of the national regional medical centers is beneficial for improving the core work ability and comprehensive quality of the trained nurses.