Objective To explore the application effect of the locking module of low temperature vaporized hydrogen peroxide sterilization program.Methods The low temperature vaporized hydrogen peroxide sterilization program locking module was added to the information system.The correct rate of sterilization program selection before the module application （January to May 2024, the operator carried out the low temperature vaporized hydrogen peroxide sterilization processor according to the routine operation process） and after the module application （June to November 2024, the low temperature vaporized hydrogen peroxide sterilization program locking module was used） and the operators′ satisfaction with the application of the program locking module were compared.Results After the application of the locking module, the correct rate of sterilization procedure selection increased from 99.88% to 100%, and the difference was statistically significant （P＜0.05）.The operators′ satisfaction regarding the application of low temperature vaporized hydrogen peroxide sterilization program locking module was high (score 4.44-4.94).Conclusion The application of the low temperature vaporized hydrogen peroxide sterilization program locking module can significantly improve the accuracy of the sterilization program, enhance operators′ satisfaction, and promote the quality management level.