Objective To explore the mediating effect of emotion regulation and experiential avoidance on the mechanism of parenting stress on parental burnout in children with autism spectrum disorder, and to provide a reference for exploring and applying intervention programs to promote the physical and mental health of parents of children with autism.Methods Based on the stress process theory, 238 parents of children with autism spectrum disorder were surveyed using the Parental Burnout Assessment, the Parenting Stress Index-Short Form, the Acceptance and Action Questionnaire and the Emotion Regulation Questionnaire, and the chain mediation model was constructed and tested.Results Parenting stress was significantly positively correlated with expressive suppression, experiential avoidance, and parental burnout, and negatively correlated with cognitive reappraisal （all P＜0.05）.Parenting stress could significantly and positively predict parental burnout, and cognitive reappraisal and experiential avoidance, expressive suppression and experiential avoidance played a partial mediating role in the effects of parenting stress and parental burnout, respectively （all P＜0.05）.Conclusion Parenting stress can impact parental burnout through the levels of cognitive reappraisal, expressive suppression, and experiential avoidance in parents of children with autism spectrum disorder.It is important to help these parents adopt positive emotional regulation strategies and coping methods to promote their mental and physical well-being.