Objective To synthesize healthcare professionals′ experiences in the de-implementation of low-value care, and to provide references for formulating targeted measures to reduce the provision of low-value care services.Methods A systematic search was conducted in PubMed, Web of Science, CINAHL, Cochrane Library, Embase, CNKI, Wanfang data, VIP, SinoMed, and Chinese Medical Journal Full-text Database for qualitative studies on healthcare professionals′ experiences of low-value care de-implementation up to March 2025.The quality of included studies was assessed using the JBI Critical Appraisal Checklist for Qualitative Research.The findings were integrated using the Meta-aggregation method.Results Twelve studies were included, from which 27 findings were extracted and categorized into 8 new categories, and further synthesized into 3 integrative findings:cognitive diffe-rences regarding low-value care de-implementation, barriers to and challenges of de-implementation, and strategies and measures for de-implementation.Conclusion Healthcare professionals exhibit varying levels of awareness regarding the de-implementation of low-value care and face multiple barriers during the process.Promotion of relevant evidence and adoption of targeted strategies are essential to facilitate the successful de-implementation of low-value care.