Objective To optimize the Outpatient Humanistic Care Experience Questionnaire and test its reliability and validity, and to provide a reliable tool for evaluating the experience of outpatient patients in hospitals. Methods Based on the previously constructed Outpatient Humanistic Care Experience Questionnaire, the questionnaire was revised through two rounds of expert consultations. A total of 598 outpatient patients from 7 hospitals in Zhengzhou were surveyed to test the reliability and validity of the questionnaire. Results The final version of the questionnaire includes 41 items across 10 dimensions:pre-hospital service process, triage process, on-site registration process, waiting process, consultation process, payment process, examination and testing process, medication pickup process, treatment process, and end of medical visit. Exploratory factor analysis identified 10 common factors with a cumulative variance contribution rate of 74.628%. The content validity index (CVI) of the questionnaire was 0.965, and the item-level content validity index (I-CVI) ranged from 0.750 to 1.000. The total questionnaire′s Cronbach′s α coefficient was 0.976, and the test-retest reliability coefficient was 0.958. Confirmatory factor analysis results showed χ2/df=2.638, RMSEA=0.052, CFI=0.954, IFI=0.915, TLI=0.899, and NFI=0.883. Conclusion The optimized Outpatient Humanistic Care Experience Questionnaire is more concise and has good reliability and validity, making it a suitable evaluation tool for assessing the humanistic care experience of outpatient patients in hospitals.