Objective To retrieve, evaluate, and integrate the best evidence on the safe management of intra-hospital transport of mechanically ventilated patients at home and abroad, so as to provide reference for standardizing intra-hospital transport. Methods Systematic retrieval of literature on the intra-hospital transport of mechanically ventilated patients from domestic and international databases and related websites. Literatures that met the inclusion criteria was screened, and its quality was evaluated and evidence was extracted. The timeframe of the search was from the construction of the database to October 2024. Results A total of 21 papers were included, consisting of 2 clinical decisions, 4 guidelines, 6 pieces of expert consensus, 7 evidence summaries and 2 systematic evaluations. Thirty-four pieces of evidence were collated and summarized into 7 aspects:pre-transfer decision-making, patient and medical staff preparation, equipment preparation, drug preparation, monitoring and treatment during transfer, emergencies and management, and transfer handover records. Conclusion The best evidence summary on the safety management of intra-hospital transport of mechanically ventilated patients provides an evidence-based basis for clinical transport practice. It is suggested that the process of intra-hospital transport should be improved from the aspects of pre-transfer decision-making, patient and medical staff preparation, etc., in order to ensure the safety of intra-hospital transport of mechanically ventilated patients.