Objective To investigate the current status of 24-hour movement behaviors and related influencing factors in stroke patients after discharge, providing a reference for clinical targeted intervention measures.Methods Using a convenience sampling method, 182 stroke patients were selected from May to December 2023.A general information questionnaire, the Barthel Index, the Nutritional Risk Screening 2002, the Perceived Social Support Scale, and the Exercise Self-Efficacy Scale were administered.Patients were required to wear an triaxial accelerometer continuously for 7 days within one week after discharge to measure their physical activity, sedentary behavior, and sleep duration, assessing whether they met the three criteria recommended by the 24-hour movement behavior guidelines.Results After discharge, stroke patients reported a daily sleep duration of （7.12±1.46） hours and a sedentary time of （5.79±2.24） hours, with moderate to vigorous physical activity time of 1.04（0.47,1.93） hours.Among the patients, 8, 23, 100, and 51 patients met 0, 1, 2, and 3 of the guideline criteria, respectively.Ordinal logistic regression analysis indicated that age, activities of daily living （ADL） independence, perceived social support, and exercise self-efficacy were influencing factors for 24-hour movement behaviors in stroke patients （P＜0.05）.Conclusion The compliance with 24-hour movement behavior guidelines in stroke patients after discharge is poor.Targeted health education should be provided by nursing staff to older patients, those with lower ADL independence, weaker social support, and lower exercise self-efficacy to improve their 24-hour movement behavior levels.