Objective To translate the Online Health-seeking Behaviour Scale (OHSB) into Chinese and validate its reliability and validity.Methods Based on the Brislin translation model, the OHSB was translated, back-translated, culturally adapted, and pre-tested to form the Chinese version of the OHSB scale.A convenience sampling method was used to conduct a questionnaire survey among 480 urban residents in September 2024 to assess the reliability and validity of the scale.Results The Chinese version OHSB scale consisted of 37 items.Exploratory factor analysis extracted three common factors, accounting for a cumulative variance contribution rate of 75.238%.The overall Cronbach′s α coefficient of the scale was 0.981, and the test-retest reliability was 0.874.The content validity index (CVI) for individual items ranged from 0.800 to 1.000, with a scale-level CVI based on the universal agreement method of 0.838 and scale-level CVI based on the average method of 0.968.The structural model fit indices were χ2/df=1.584, RMSEA=0.054, TLI=0.935, and CFI=0.939.Conclusion The Chinese version of the OHSB demonstrates good reliability and validity and can be used as an effective tool for assessing the level of online health-seeking behavior among the public.