Objective To summarize the best evidence of exercise intervention for children with autism spectrum disorder, and to provide evidence-based practical reference for exercise rehabilitation training. Methods According to the "6S" pyramid model, a systematic literature search was performed in domestic and international databases as well as related websites. Publications dealing with exercise intervention for children with autism spectrum disorder published up to June 2024 were included.A study selection and the critical appraisal of the selected studies were performed independently by two reviewers. Then evidence was extracted, summarized and rated. Results A total of 15 studies met the inclusion criteria, including 3 best practice reports, 1 guideline, 1 evidence summary, 4 systematic reviews, 2 meta-analyses, and 4 randomized controlled trials.Finally, 19 pieces of best evidence were formed in 6 aspects: exercise intervention team, exercise intervention principle, exercise frequency, exercise type, relevant intervention methods and precautions. Conclusion The best evidence of exercise rehabilitation training for children with autism spectrum disorder can provide practical guidance for carrying out scientific, standardized and unified exercise intervention.