Objective To explore the level of humanistic care experience of ophthalmic outpatients and analyze its influencing factors, aiming to provide reference for taking targeted interventions to enhance treatment-seeking experience of this population. Methods A total of 992 ophthalmic outpatients were invited to complete the Outpatient Experience of Human Caring Scale (OEHC-Scale). Results The total OEHC-Scale score in ophthalmic outpatients was (203.61±25.93). Monthly income per household member, education level, disease type, clarity of the patient flow at the outpatient clinic and location of the hospital were the factors influencing humanistic care experience of ophthalmic outpatients (all P<0.05). Conclusion Humanistic care experience of ophthalmic outpatients is relatively high. Low family income, low education level, organic ophthalmologic problem and unclear patient flow process would reduce the humanistic care experience of ophthalmic patients. Hospital administrative departments should take targeted interventions to improve the humanistic care experience of ophthalmic outpatients.