Objective To develop and provide a new evaluation tool to comprehensively understand the knowledge, attitudeand practice (KAP) of palliative care for nurses. Methods With the theoretical model of knowledge-attitude-practice as the guiding framework, the first draft of the questionnaire was formed after literature review, group discussion and Delphi expert correspondence. Questionnaire survey was conducted to analysis the item and reliability and validity of the tool among the 575 nursing staffs of 10 general hospitals in Hubei Province by the convenience sampling method. Results There were 39 items in the KAP questionnaire on hospice and palliative care for nurses. Three common factors were extracted by exploratory factor analysis, and the cumulative variance contribution rate was 80.885%. The fitting indicators of confirmatory factor analysis were all within the acceptable range (χ2/df=2.835, RMSEA=0.078, IFI=0.918, CFI=0.918, TLI=0.912), the Cronbach′s α was 0.976, and the half-time reliabilitywas 0.808; the content validity indexs of each item of the questionnaire were 0.875-1,the average scale-level content validity index was 0.965. Conclusion The KAP questionnaire on hospice and palliative care for general hospital nurses has good reliability and validity, and can be used as an effective tool to evaluate the knowledge, belief and practice of general hospital nursesfor palliative care.