Objective To explore the effects of a virtual healing environments intervention based on virtual reality technology on alleviating perceived stress and symptom burden in hemodialysis patients.Methods A total of 52 hemodialysis patients were randomly assigned to a control group and an intervention group, with 26 patients in each, then the two groups were assigned to two different wards.The control group received routine hemodialysis care, while the intervention group additionally received virtual healing environments intervention during hemodialysis, three times a week for four weeks.Perceived stress and symptom burden before the intervention, at the end of the fourth week of the intervention, and one month after the intervention were assessed and compared between the two groups.Results A total of 25 patients in the intervention group and 26 patients in the control group completed the study and follow-up.There were statistically significant differences in the total scores of perceived stress and symptom burden between the two groups, with time effects, group effects, and time × group interaction effects being significant （all P＜0.05）.Conclusion Implementing virtual healing environments intervention for hemodialysis patients provides a diverse, accessible, repetitive, and standardized therapeutic environment, it can effectively reduce patients′ perceived stress and symptom burden, and improve their mental health.