Objective To systematically retrieve the relevant evidence on the prevention and management of arteriovenous graft （AVG） infection in hemodialysis patients, and to provide reference for clinical practice.Methods Clinical decision support statement, guideline, systematic review, evidence summary, expert consensus report, and original research were searched in evidence-based resources, guideline websites, websites of professional societies or organizations, and bibliographic databases from database inception until January 22, 2024.Results A total of 16 publications were eligible, including 9 guidelines, 2 expert consensus reports, 1 evidence summary, 2 clinical decision support statements, 1 systematic review, and 1 cohort study. Thirty-three pieces of best evidence were summarized into 6 aspects:risk factors, perioperative infection prevention and control, standardized puncture procedures, health education for daily maintenance, identification and treatment of infection, and continuous quality improvement.Conclusion This study provides the best evidence for the prevention and management of AVG infection.It is recommended that healthcare professionals should consider specific clinical situations when translating the evidence into practice, and develop indivi-dualized infection prevention and management protocols, in an effort to decrease AVG infection.