This paper summarizes experience of course construction of provincial first-class Nursing Humanistic Cultivation in baccalaureate nursing education. The study mainly elaborates six characteristics of the course:collaboration between hospital and nursing school to share teaching resources, colorful extracurricular activities, multiple teaching methods, selecting national planning textbook, multi-round and whole-process evaluation, integrating with curriculum-based ideological and political education.The effect is expounded according to the development of students and teachers, and the perspectives on further practice are put forward, aiming to provide reference for other similar nursing schools and to improve the quality of nursing humanistic education.