Objective To describe the level and influencing factors of self-regulatory fatigue among pregnant women with gestational diabetes mellitus（GDM）, and to provide reference for targeted intervention.Methods A total of 318 pregnant women with GDM were investigated using a sociodemographic questionnaire, the Self-regulatory Fatigue Scale, the General Self-efficacy Scale, the Connor-Davidson Resilience Scale and the Social Support Rating Scale.Results The sample scored （50.44±8.23） for self-regulatory fatigue.Multiple linear regression analysis showed that whether work or home, average monthly household income per capita, weeks of pregnancy, self-efficacy, resilience and social support were factors affecting self-regulatory fatigue （all P＜0.05）, which could explain 54.1% of the total variance.Conclusion Pregnant women with GDM report high level of self-regulatory fatigue.Medical staff should identify high-risk women, and pay more attention to those who attend work, those with low family income and with ≥28 weeks of pregnancy.Interventions should be carried out to improve their self-efficacy, resilience and social support, in an effort to alleviate their self-regulatory fatigue.