Objective To explore the effectiveness of the multi-scenario work style practice teaching for the elderly in vocational nursing specialty, and to provide reference for the cultivation of nursing professionals with high quality technical skills. Methods Four classes of nursing students of grade 2022 were randomly divided into two groups according to their classes. The control group (n=93) implemented regular practice teaching, and the four courses of Basic Nursing, Internal Medicine Nursing, Surgical Nursing, and Geriatric Nursing were carried out in-class and comprehensive practice according to the curriculum standards.The observation group (n=89) implemented the multi-scenario work style practice teaching for the elderly, for example, the core skills of the four courses were integrated in a case-based manner according to the semester and the disease progression of the elderly, formed a multi-scenario task chain focused on hospitals, medical care institutions, and community home care, so as to realize the practice teaching of holistic care for the elderly. At the end of the course, the two groups were evaluated for practical operation achievements and humanistic care ability, and the observation group′s evaluation of satisfaction with the practical teaching was also investigated. Results At the end of the course, the scores of the practical operation achievement and humanistic care ability in the observation group were significantly higher than those in the control group (both P<0.05); there were 95.5% of the nursing students in the observation group recognized the multi-scenario work style practice teaching for the elderly, and 91.0%-98.9% of the students believed that it could improve the ability and efficiency of practical operation, the ability of health education, and the ability of analyzing and solving problems and so on. Conclusion Adopting the multi-scenario work style practice teaching for the elderly can improve the practical ability and humanistic care ability of nursing students.