Objective To explore the effects of upright delivery on the duration of labor and delivery outcomes of natural childbirth.Methods A total of 481 women with normal labor and vaginal delivery admitted to New Century Women′s and Children′s Hospital from January 2021 to June 2023 were selected as the study objects.The control group was delivered in the traditional supine position (n=339), while the observation group was delivered in the upright position (n=142).Differences in duration of labor, degree of perineal laceration, postpartum hemorrhage, and pH value, base remaining (BE), and lactate of the neonatalumbilical artery blood gas were compared between the primipara and multiparous women within the two groups, respectively. Results The time of the first stage of labor, the second stage of labor and the total labor in the observation group was significantly shorter than those in the control group (all P<0.05). The episiotomy rate in primiparas in the observation group was significantly lower than that in the control group (P<0.05), and there were no statistically significant differences in the degree of perineal laceration and postpartum hemorrhage between the two groups (both P>0.05).The pH value, BE, and lactate of neonatal umbilical artery blood gas in primiparas and the pH value of neonatal umbilical artery blood gas in multiparous in the observation group were better than those of the control group, and the differences were statistically significant (all P<0.05).Conclusion The upright delivery method may assist women in natural delivery to shorten the duration of labor, reduce the incidence of neonatal acidosis, promote thenatural delivery, and decrease the risk of adverse outcomes for both mothers and infants.