Objective To translate the best evidence regarding intravenous insulin therapy for ICU patients into practice, and to eva-luate its effectiveness.Methods Guided by the evidence implementation model, the best evidence summarized was translated into 12 audit indicators and baseline audit was conducted, then barriers and facilitators were identified, and reform was made.The execution rate of audit indicators and knowledge of medical staff toward intravenous insulin infusion were compared before and after evidence implementation.Meanwhile, blood glucose level, incidence of hyperglycemia and hypoglycemia, as well as percentage coefficient of variation for glucose （%CV） in patients were also compared.Results After implementing the best evidence, the execution rates of 10 audit indicators were significantly enhanced （all P＜0.05）.The total score of knowledge of medical staff was also improved from （47.87±14.76） points to （88.32±9.56） points, with a statistically significant difference （P＜0.05）.Patients′ blood glucose level, the incidence of hyperglycemia, and proportion of high %CV were significantly reduced after evidence implementation （all P＜0.05）.Conclusion Integrating the best evidence into clinical practice for intravenous insulin infusion in ICU patients, can improve medical staff′s knowledge and behavior of intravenous insulin therapy, which result in stabilizing patients′ blood glucose.