Objective To explore the mediating effect of clinical sense of belonging between workplace adaptation and humanistic practice ability of clinical nursing interns, and to provide a reference for nursing educators to improve the humanistic practice ability of clinical nursing interns.Methods A total of 300 clinical nursing interns were investigated by the general demographic information questionnaire, Belongingness Scale-Clinical Placement Experience (BES-CPE), Nurses′ Workplace Adaptability Scale (NWAS) and the Nurses′ Humanistic Practice Ability Measurement Scale.Results The score of BES-CPE, NWAS and humanistic practice ability among clinical nursing interns were 103.65±18.46, 67.58±12.24, and 108.78±18.03, respectively.There was a positive correlation between clinical sense of belonging, workplace adaptability and humanistic practice ability of intern nursing students (all P＜0.05), and the percentage of the mediating effect of clinical sense of belonging between workplace adaptability and humanistic practice competence was 61.52%.Conclusion Clinical sense of belonging of clinical nursing interns plays a partial mediating role in workplace adaptation and humanistic practice ability, and humanistic practice ability can be improved by improving clinical sense of belonging and workplace adaptation of clinical nursing interns.