Objective To explore the current status and influencing factors of kinesiophobia in patients after anterior cruciate ligament reconstruction, so as to provide a reference for developing targeted nursing intervention. Methods A total of 240 patients after anterior cruciate ligament reconstruction were randomly selected, then they were investigated by using a general information questionnaire, the Tampa Scale for Kinesiophobia (TSK), the Hospital Anxiety and Depression Scale (HADS), the Mindful Attention Awareness Scale (MAAS), the Visual Analogue Scale (VAS) and Pittsburgh Sleep Quality Index (PSQI). Univariate analysis and multivariate logistic regression analysis were conducted to identify its major influencing factors. Results Totally 106 patients (44.17%) experienced kinesiophobia. Logistic regression analysis showed that,the scores of MAAS, VAS, depression, PSQI and BMI were the major influencing factors of kinesiophobia in patients (all P<0.05). Conclusion The incidence of kinesiophobia in patients after anterior cruciate ligament reconstruction is high, which is easily affected by psychological state, BMI, pain degree and sleep quality. It is necessary to strengthen psychological intervention and pain management for this population, and improve their sleep quality, in order to reduce the level of kinesiophobia, and promote their rehabilitation of keen joint function.