Objective To understand nursing students′ experience in the use of large language model chatbots, and to provide a reference for standardized usage of large language model chatbots. Methods Descriptive qualitative research approach was adopted. Using purposive sampling strategies, online semi-structured interviews were conducted with 15 nursing students from 4 medical universities. Content analysis method was used to analyze, summarize, and extract themes from the interview data. Results A total of 4 themes were extracted: diverse experiences of usage； differentiation of usage scenarios； potential issues of usage； self-perceived needs and expectations. Conclusion Nursing educators and managers should lead nursing students to establish correct values towards the usage of large language models, develop relevant guidelines to standardize their usage, and must strengthen training and nursing ethics construction, in an effort to promote the innovative integration of large language models in nursing education.