Objective To construct systematic indicators for the development of clinical practice training bases for anesthesia nurse specialists, and to provide a reference for the cultivation of anesthesia nurse specialists and the construction of training bases. Methods Based on the principles of evidence-based practice and site surveys and expert panel discussions, indicators for the development of clinical practice training bases for anesthesia nurse specialists were drafted. The Delphi method was used to conduct two rounds of expert inquiries with 22 experts, and the Analytic Hierarchy Process (AHP) was applied to determine the weights of each level of indicator. Empirical research was conducted to verify the discriminability of the indicators. Results The response rate for two rounds of expert consultation was 100%, with the authority coefficient of the experts being 0.941 and the Kendall′s concordance coefficients for the first, second, and third-level indicators being 0.654, 0.486, and 0.671, respectively (all P<0.05). The final indicators for the clinical practice training bases for anesthesia nurse specialists included 5 primary indicators, 14 secondary indicators, and 50 tertiary indicators. Experts used these indicators to assess 14 clinical practice training bases for anesthesia nurse specialists in Guangdong province, and the results showed that these indicators showed good discriminability. Conclusion The indicators for constructing clinical practice training bases for anesthesia nurse specialists are clear in hierarchy and high in discriminability, demonstrating scientific and practical value. They can standardize the cultivation of anesthesia nurse specialists and the construction of training bases.