Objective To introduce the Animated Activity Questionnaire (AAQ) and test its reliability and validity in patients with knee osteoarthritis. Methods AAQ was translated into Chinese through forward translation, back translation, cultural adaptation and pre-survey to form the Chinese version of the Animated Activity Questionnaire. A total of 326 patients with knee osteoarthritis were selected for questionnaire survey to evaluate the reliability and validity of the questionnaire. Results The Chinese version AAQ consisted of 17 items which were factored into two dimensions:gait and walking ability, daily movement behavior capacity. Exploratory factor analysis extracted two common factors with a cumulative variance contribution rate of 71.145%. Confirmatory factor analysis showed the following results:χ2/df=2.976，RMSEA=0.095，NFI=0.917，IFI=0.943，TLI=0.931，CFI=0.943，GFI=0.856, indicating acceptable overall model fit. The content validity index at the item level ranged from 0.833 to 1.000, and stood at 0.971 at the scale level. The Cronbach′s α coefficient for the questionnaire was 0.954, and the Guttman split-half reliability coefficient was 0.865. Conclusion The Chinese version AAQ demonstrates good reliability and validity, making it a suitable assessment tool for evaluating activity limitations in patients with knee osteoarthritis in Chinese cultural context.