Objective To evaluate the effects of hospice care training based on the concept of participatory and interactive teaching in undergraduate nursing interns. Methods Undergraduate nursing interns were recruited voluntarily and divided into an intervention group (n=71) and a control group (n=44) according to whether they were willing to participate in hospice care training. The control group received routine clinical practice and hospice care teaching, while the intervention group received hospice care training based on the concept of participatory and interactive teaching besides routine clinical practice. The training effect was evaluated after clinical practice. Results After the training, the score of the Frommelt Attitudes Toward Care of the Dying Scale Form Bin the intervention group was significantly higher than that of the control group (P<0.05).The death attitude of the interns in the two groups tended to be neutral acceptance, and there wasn′t statistically significant difference between the groups (P>0.05). After the training, the interns in the intervention group were satisfied or basically satisfied with their training effect. Conclusion The participatory and interactive hospice care training is recognized by the undergraduate nursing interns,and it is conducive to improving the attitudes of undergraduate nursing interns toward the care of the dying and helping them face patients′ death properly.