Objective To explore the effect of applying online and offline experiential teaching in ideological and political education of Introduction to Nursing course. Methods Convenience sampling method was used to select 2 nursing undergraduate classes of 2022 as the experimental group, and a combination of online and offline experiential teaching mode was applied. Two undergraduate nursing classes of 2021 were selected as the control group, and subjected to traditional teaching mode. At the end of the course, the two groups of students were compared in terms of written exam scores, ideological and political effects of the course, and students′ satisfaction with the teaching mode and the improvement of comprehensive quality. Results After the teaching, the written exam scores of the experimental group were (83.83±5.84) points, versus (80.94±5.56) points in the control group (P<0.05). The proportions of students in the experimental group who rated the ideological and political effects as obvious were significantly higher than those in the control group; the experimental group had significantly higher satisfaction with the teaching mode and comprehensive quality improvement than the control group (all P<0.05). Conclusion The combination of online and offline experiential teaching can obviously improve the ideological and political effect of the curriculum, and enhance student′s comprehensive quality.