We summarized the nursing experience of a patient with Creutzfeldt-Jakob disease complicated by purple urine bag syndrome. Key nursing points included early observation and care of muscle spasms, identification and management of purple urine bag syndrome, implementation of personalized nutritional support, enforcement of isolation and protective measures, preparation of home protection and medication guidance before discharge, strict verification of all items used by the patient after discharge, and terminal disinfection. Through multidisciplinary collaborative treatment and meticulous care, no serious complications occurred during the patient′s hospitalization period, and the family requested discharge 15 days after a clear diagnosis. A telephone follow-up was conducted one month after discharge, and no pressure ulcers, lung infections, or reoccurrence of purple urine bag syndrome were reported for the patient.