Objective To evaluate the effect of digital health technology on negative emotions and turnover intention in clinical nurses.Methods Electronic databases such as PubMed, Web of Science, Cochrane Library, China National Knowledge Infrastructure (CNKI), WanFang, VIP, etc.were searched from database inception until October 2023 to retrieve RCT studies reporting the effect of digital health technology on negative emotions in nurses.Meta-analysis was performed using RevMan5.3.Results A total of 17 studies were included, involving 2 285 nurses.Compared with the control group, digital health technology could significantly reduce nurses′ depression and anxiety, improve sleep quality, decrease stress level and turnover intention（all P＜0.05）.The effect on post-traumatic stress disorder was not obvious.Conclusion The present meta-analysis supports that the use of digital health technology has a beneficial effect on the negative emotions and turnover intention in clinical nurses, but the effect on post-traumatic stress disorder needs to be further verified.