Objective To investigate the status quo of nurses′ self-regulated fatigue and perceived hospital magnetic level, explore the effect of hospital magnetic level on nurses′ self-regulated fatigue, so as to provide a relevance to formulating nursing intervention. Methods From May to August 2023, 825 clinical nurses in 2 Class A tertiary hospitals in Xi′an were investigated by general data questionnaire, Essentials of Magnetism Scale and Self-regulating Fatigue Scale. Results The score of self-regulated fatigue was 48.22±6.62, perceived hospital magnetic level was 103.44±14.84. Hospital magnetic level was negatively correlated with nurses′ self-regulated fatigue. Hospital magnetic level, type of employment, monthly income and night shift frequency were the main influencing factors of nurses′ self-regulated fatigue (all P＜0.05). Hospital magnetic level could explain the total variation of nurses′ self-regulated fatigue by 16.00%. Conclusion Nurses′ self-regulated fatigue and perceived hospital magnetism levels were in the middle, and hospital magnetism level was an important factor affecting nurses′ self-regulated fatigue.Managers can reduce nurses′ self-regulating fatigue by improving hospital magnetic levels.