Objective To explore the effect of applying multi-disciplinary integrated classroom teaching in clinical teaching for nursing interns working in orthopedic department. Methods A total of 96 undergraduate nursing students who were having clinical internship in the orthopedics department were selected and evenly divided into 2 groups according to the order of department entry for internship. The control group was subjected to a conventional clinical nursing practice teaching mode, while the experimental group was additionally subjected to a multi-disciplinary integrated classroom teaching mode. Department exit exam scores, teaching effectiveness, and critical thinking skills were compared between the two groups. Results The experimental group had significantly higher exit exam scores, better teaching effect evaluation and critical thinking capability scores (all P＜0.05 ). Conclusion Applying multi-disciplinary integrated classroom teaching in clinical teaching could improve nursing students′ critical thinking capability, thus enhancing teaching effect.