Objective To explore the effect of optimizing the practice training program for specialist nurses in pelvic floor rehabilitation based on action research.Methods Based on the theory of action research, three rounds of action adjustment and optimization of the training program were conducted to address the problems of the clinical practice of 35 (10, 12, and 13 in the three rounds) specialist nurses in pelvic floor rehabilitation, and the training program was evaluated based on the Kirkpatrick model.Results The demand-oriented clinical practice training program for specialist nurses in pelvic floor rehabilitation was developed.In the three rounds of action research, the overall satisfaction rates of the trainees with the clinical practice training were 90.0%, 91.7%, and 100.0%, respectively.After the training, the scores of the trainees′ (a total of 25 in the 2nd and 3rd rounds) mastery of pelvic floor related knowledge and skills were significantly higher than those before the training (all P＜0.05), and more than 80% of the trainees thought that they could apply the pelvic floor related professional knowledge and skills in clinical practice.Conclusion The action research is conducive to optimizing the clinical practice training program for specialist nurses in pelvic floor rehabilitation, thus improving the training effect and trainee satisfaction, and positively affecting the trainees′ clinical work.