Objective To explore the effectiveness of nurse informatics competence training based on the application program of End User Computing (EUC) strategy in enhancing nurses′ informatics competence. Methods A training guidance team was established to provide a course on application program design for 33 nurses based on the EUC strategy, utilizing programming tools. The effectiveness of the training was evaluated using the Nurse′s Informatics Competency Scale, Course Learning Evaluation Questionnaire, and information project outputs.Results After the training, the overall scores for nurses′ computer skills, information literacy, and informatics competency significantly improved (all P<0.05). The overall score for the course satisfaction was 81.48±12.91, with satisfaction ratings in course content, knowledge, attitude, and project management being above average. A total of 9 information application program design projects were generated by 9 nurse groups. Conclusion Nurses can cultivate their information thinking, improve informatics capabilities, and achieve a high level of course satisfaction through application program design courses.