Objective To construct an evaluation index system of information security literacy for clinical nurses, so as to provide references for training and evaluation of clinical nurses. Methods Based on Knowledge-Attitude-Practice (KAP) theory, the draft of evaluation index system and consultation questionnaire were compiled by literature research and field investigation. Two rounds of expert consultation were conducted with 20 experts across the country and Analytic Hierarchy Process was used to determine the weights of indicators of all levels. Results The response rates of two rounds of expert consultation were both 100%; the authority coefficients were 0.945 and 0.950 respectively; the Kendall′s coefficients of concordance for all indicators were 0.179 and 0.193 respectively (both P.<0.05) .The final evaluation index system of information security literacy for clinical nurses consisted of 3 first-level indicators (information security knowledge, information security awareness and information security behaviors),12 second-level indicators and 59 third-level indicators. Conclusion The evaluation index system of information security literacy for clinical nurses is comprehensive, scientific and reliable in contents，which can provide an objective quantitative basis for information security training and assessment of clinical nurses.