Objective To explore the application effect of virtual simulation technology combined with rain class in the practice teaching of enterostomy nursing, in order to provide a reference for improving nursing practice teaching quality. Methods The baccalaureate nursing students from two natural classes and enrolled in 2020 were randomized into a control group (n=37) and an experimental group (n=36). The traditional practice teaching model was used in the control group, which consisted of teacher de-monstration, students practice, teacher touring duidance and student demonstration in order, while a new teaching model of virtual simulation technology combined with rain class was adopted in the experimental group. The theory and skill examination scores of the two groups were compared and the teaching effect was evaluated. Results After the teaching, the theory and skill examination scores of the experimental group were significantly higher than those of the control group (both P<0.05), and more than 70% students in the experimental group recognized their teaching model, and gave full affirmation to its teaching effect. Conclusion Virtual simulation technology combined with rain class helps students strengthen slef-directed learning ability, stimulate learning interest and cultivate clinical thinking, so it can improve the quality of enterostomy nursing practice teaching.