Objective To examine the effect of fluid management education based on mixed reality technology in adolescents with neurogenic bladder. Methods Eighty adolescents with neurogenic bladder were randomized into two groups, with 40 in each.The control group received routine fluid management education using booklet, while the intervention group used mixed reality technology for education.Fluid management compliance, selfmanagement ability, parent satisfaction with health education and the incidence of urinary tract infection （UTI） within two months after discharge were compared between the two groups. Results After the intervention, the intervention group had significantly higher fluid management compliance, selfmanagement ability and parent satisfaction, compared with the control group (all P＜0.05).The incidence of UTI was 20.00% in the intervention group and 27.50% in the control group, without significant difference (P＞0.05). Conclusion Health education based on mixed reality technology is helpful to improve healthrelated behaviors and parent satisfaction in adolescents with neurogenic bladder, and it is possible to relatively reduce UTI within two months of discharge.