Objective To systematically evaluate the effect of Virtual Reality (VR) on the rehabilitation of patients with Mild Cognitive Impairment (MCI), and to provide a reference for conducting standardized and effective rehabilitation training. Methods Randomized Controlled Trials studies (RCTs) concerning the intervention effect of VR on patients with MCI were retrieved from the electronic databases of Web of Science, PubMed, Cochrane Library, Embase, CNKI, WanFang Data, VIP and SinoMed from the establishment of the database to December 2022.Then Meta analysis was performed using the RevMan 5.4 software. Results Sixteen RCTs were included, involving 747 participants.Meta analysis results indicated that, compared with conventional cognitive training or usual care measures, VR cognitive training improved overall cognitive function in patients with MCI ［SMD=0.97,95%CI（0.44,1.50）,P＜0.05］,subgroup analysis showed that, intervention course ＜8 weeks ［SMD=1.79,95%CI（0.50,3.00）,P＜0.05］,total duration ≥15 hours ［SMD=1.49,95%CI（0.64,2.33）,P＜0.05］,semi-immersive VR ［SMD=3.59,95%CI（3.34,3.85）,P＜0.05］ had significantly better effects;VR cognitive training enhanced executive function ［SMD=-0.89,95%CI（-1.62,-0.15）,P＜0.05］, attention ［SMD=0.81,95%CI（0.24,1.39）,P＜0.05］, and quality of life ［SMD=0.61,95%CI（0.33,0.88）,P＜0.05］, while there wasn′t significant difference in memory function ［SMD=0.38,95%CI（-0.19,0.95）,P＞0.05］. Conclusion VR technology can improve the overall cognitive function of MCI patients.A semi-immersive VR intervention is recommended, with an intervention period of less than 8 weeks and a total duration of at least 15 hours.VR also enhances patients′ executive function, attention, and quality of life, but it does not improve memory function.