Objective To compare the application effects of different venous accesses in high pressure injection of CT enhanced scan contrast media, so as to provide evidence for selecting appropriate venous access for cancer patients. Methods A total of 200 cancer patients undergoing CT enhanced scan were divided into three groups according to different venous accesses used for high pressure injection of contrast media, which were 22G （Bard） indwelling needle (indwelling needle group, n=88), the high-pressure resis-tant injection needle infusion port (infusion port group, n=55), and the high-pressure resistant injection PICC catheter (PICC group, n=57).All the participants were injected loversol injection by using high pressure injection pump, with the maximum pressure was no more than 324 psi, and the flow rate was 1.6-2.5 mL/s.The injection flow rate, the venous access pressure, the length of contrast media injection period, the pain at the injection site and the quality of image scanning were recorded and compared among the three groups. Results There were significant differences in the injection flow rate, the venous access pressure, the length of contrast media injection period, the pain at the injection site and the quality of image scanning among the three groups (all P＜0.05）.In the indwelling needle group, the injection flow rate was the highest ［（2.30±0.16）mL/s］, the pressure was the lowest ［（48.58±3.15） psi］, the length of injection period was the shortest ［（35.12±4.88）s］, while the pain at the injection site was the mildest (rank mean 70.50), and its quality of image scanning was the best (rank mean 73.51). Conclusion In CT enhanced positioning of high-pressure intravenous injection of contrast media, the application effect of 22G indwelling needle is better than using PICC and infusion port.