Objective To observe the therapeutic effect of Kangfuxin combined with Saifurun on acute radiation-induced skin injury in rats. Methods A total of 40 SD rats were selected, then radionuclide 32P was fixed on their back skin to prepare an acute radiation skin injury model.After 2 weeks of modeling, the rats were randomized into a model group, a Kangfuxin group, a Saifurun group, and a Kangfuxin+Saifurun group, with 10 rats in each group.In the model group, the skin lesions were wiped with normal saline, the skin lesions were smeared with Kangfuxin in the Kangfuxin group, the skin lesions were sprayed with Saifurun in the Saifurun group, and in the Kangfuxin+Saifurun group, the skin lesions were smeared with Kangfuxin first, then sprayed with Saifurun twice a day after drying.Each group received medication at 8:00 and 16:00 every day.The local changes and healing time of the wound were observed.The skin lesions were taken for H-E staining and Masson staining at the 2nd, 4th and 8th week of treatment. Results When compared with the model group, the healing time of the other three groups were significantly shorter （all P＜0.05）, and the healing time of the Kangfuxin+Saifurun group was significantly shorter than the Kangfuxin group and the Saifurun group （both P＜0.05）.The pathological examination results showed that, the degree of inflammatory cell infiltration and edema in the wound tissue of the combined group were significantly lower than that of the other groups, and the number of new capillaries and collagenous fiber content were significantly higher than those of the other groups, its collagenous fibers were arranged more neatly and the epidermal structure was more complete.When compared with the model group, the collagenous fiber content in the other three groups were higher at the 2nd, 4th and 8th week of treatment （all P＜0.05）, and the collagenous fiber content in the Kangfuxin+Saifurun group was higher than those in the Kangfuxin group and the Saifurun groups （both P＜0.05）. Conclusion Kangfuxin combined with Saifurun can alleviate the early inflammatory reaction of the radiation-induced skin injury, promote the angiogenesis and collagenous fiber formation of the wound, shorten the wound healing time, and accelerate the wound repair.