Objective To deeply understand barriers of nurses applying the best evidence to prevent Intraoperative Acquired Pressure Injury (IAPI), so as to provide a reference for implementing pressure injury intervention. Methods A descriptive qualitative study method was adopted.A total of eleven nurses from the operating room of 6 Grade A hospitals in western China were selected, then the interview outline was formulated based on the Theoretical Domain Framework to conduct semi-structured deep interview, and the directional content analysis method was used to induce and refine themes. Results Barriers to application of best evidence among nurses included knowledge (lack of relevant knowledge about the best evidence), environmental factors (lack of positioning aids for special surgery, poor intelligence of hospital information system), motivation and goals (patients and their families′ less attention to IAPI prevention affected the enthusiasm of operating room nurses), code of conduct (workflow should be improved), outcome expectations (operating room nurses lacked confidence in the effectiveness of carrying out best evidence). Conclusion There are va-rious barriers affecting the clinical application of best evidence on IAPI prevention, and it is necessary for operating room to identify these barriers, then promote the clinical application of the best evidence on IAPI prevention.