Objective To investigate the current status and explore the influencing factors of sedentary behavior among patients with colorectal cancer during the intermittent period of chemotherapy, and to references for improvement of physical activity level in this population. Methods A total of 200 patients with colorectal cancer during the intermittent period of chemotherapy were selected by convenience sampling method, and were surveyed by using the general information questionnaire, the Sedentary Behavior Questionnaire （SBQ）, the Falls Efficacy Scale-International （FES-I）, the Exercise Barriers Scale, and the Family APGAR Index. Results Colorectal cancer patients had a median sedentary time of 7.6（IQR 5.5-8.6） h/d during the intermittent period of chemotherapy, with 136 （68.00%） having a sedentary time ≥6 h/d.Logistic regression analysis showed that, age, chemotherapy cycles, pain, fall efficacy, family functionality were influencing factors of sedentary behavior of patients with colorectal cancer during the intermittent period of chemotherapy (all P＜0.05）. Conclusion Colorectal cancer patients during the intermittent period of chemotherapy engaged in long time of sedentary behavior.Medical staff should take intervention measures targeting the risk factors of sedentary behavior, in an effort to lessen patients′ sedentary behavior.